Journal: Open Veterinary Journal

Article Title: Diagnostic utility of measuring lactate dehydrogenase levels and its isoenzyme activities for the evaluation of malignancy in feline pleural effusion and ascitic fluid

doi: 10.5455/OVJ.2022.v12.i5.19

Figure Lengend Snippet: Comparison of LDH levels and LDH isoenzymes activities among the malignant group, the NMNI group, and the infectious group

Article Snippet: LDH levels and LDH isoenzyme subgroup activities were measured in all samples (LSI Medience Co., Tokyo, Japan).

Techniques: Comparison

Journal: Open Veterinary Journal

Article Title: Diagnostic utility of measuring lactate dehydrogenase levels and its isoenzyme activities for the evaluation of malignancy in feline pleural effusion and ascitic fluid

doi: 10.5455/OVJ.2022.v12.i5.19

Figure Lengend Snippet: Comparison of LDH isoenzyme activities in PE and AF among the malignant, NMNI, and infectious groups. Box plots show the median, range, and 25th and 75th quartiles within the groups. LDH level, LDH-3, and LDH-4 activities were significantly higher in the malignant group than those in the NMNI group ( p = 0.007, 0.007, and 0.002, respectively). LDH-1 activity was significantly lower in the malignant group than that in the NMNI group ( p < 0.001). LDH-1 activity was significantly lower in the malignant group than that in the infectious group ( p = 0.031). LDH level and LDH-4 activity were lower in the malignant group than those in the infectious group ( p = 0.015, respectively). Statistical analyses were performed using the Kruskal–Wallis test. Post hoc comparisons were conducted using the Mann–Whitney U test with Bonferroni correction. * p < 0.05, ** p < 0.01; LDH, lactate dehydrogenase; PE, pleural effusion; AF, ascitic fluid; NMNI, non-malignant, non-infectious.

Article Snippet: LDH levels and LDH isoenzyme subgroup activities were measured in all samples (LSI Medience Co., Tokyo, Japan).

Techniques: Comparison, Activity Assay, MANN-WHITNEY

Journal: Hereditas

Article Title: RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4

doi: 10.1186/s41065-025-00453-0

Figure Lengend Snippet: Deficiency of RBM15 ameliorated H/R-stimulated oxidative damage in AC16 cells. AC16 cells were treated with H/R, H/R + si-NC or H/R + si-RBM15 and untreated cells were control. ( A and B ) The mRNA and protein levels in AC16 cells were measured by qRT-PCR assay and western blot assay, respectively. ( C ) AC16 cell viability was explored by CCK-8 assay. ( D - G ) The levels of LDH, ROS, MDA and SOD in AC16 cells were examined by related commercial kits. ** P < 0.01, *** P < 0.001

Article Snippet: LDH level in AC16 cells was measured using Promega CytoTox 96 ® kit (Promega, Madison, WI, USA) in line with the protocols.

Techniques: Control, Quantitative RT-PCR, Western Blot, CCK-8 Assay

Journal: Hereditas

Article Title: RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4

doi: 10.1186/s41065-025-00453-0

Figure Lengend Snippet: RBM15 knockdown repressed H/R-induced AC16 cell damage by altering ACSL4 expression. ( A ) The protein level of ACSL4 in AC16 cells transfected with pcDNA-NC or pcDNA-ACSL4 was measured by western blot. ( B - K ) AC16 cells were divided into 5 groups: control, H/R + si-NC, H/R + si-RBM15, H/R + si-RBM15 + pcDNA-NC and H/R + si-RBM15 + pcDNA-ACSL4. ( B ) AC16 cell viability was assessed by CCK-8 assay. ( C - G ) The levels of LDH, ROS, MDA, SOD and Fe 2+ in AC16 cells were examined by commercial kits. ( H ) The protein levels of GPX4, ACSL4, FTH1 and NCOA4 in AC16 cells were measured by western blot. ( I - K ) The levels of GSH, GSSG and GSH/GSSG in AC16 cells were examined by the indicated kits. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: LDH level in AC16 cells was measured using Promega CytoTox 96 ® kit (Promega, Madison, WI, USA) in line with the protocols.

Techniques: Knockdown, Expressing, Transfection, Western Blot, Control, CCK-8 Assay

Journal: SpringerPlus

Article Title: Highly improved accuracy of the revised PREoperative sarcoma score (rPRESS) in the decision of performing surgery for patients presenting with a uterine mass

doi: 10.1186/s40064-015-1318-7

Figure Lengend Snippet: Positive findings in patients

Article Snippet: In our previous report (Nagai et al. ), univariate analyses revealed that age, serum LDH levels, MRI findings, and endometrial cytology findings were significant factors in the preoperative prediction of uterine sarcoma.

Techniques:

Journal: SpringerPlus

Article Title: Highly improved accuracy of the revised PREoperative sarcoma score (rPRESS) in the decision of performing surgery for patients presenting with a uterine mass

doi: 10.1186/s40064-015-1318-7

Figure Lengend Snippet: Multivariate analysis of statistically significant positive findings

Article Snippet: In our previous report (Nagai et al. ), univariate analyses revealed that age, serum LDH levels, MRI findings, and endometrial cytology findings were significant factors in the preoperative prediction of uterine sarcoma.

Techniques:

Journal: SpringerPlus

Article Title: Highly improved accuracy of the revised PREoperative sarcoma score (rPRESS) in the decision of performing surgery for patients presenting with a uterine mass

doi: 10.1186/s40064-015-1318-7

Figure Lengend Snippet: Parameter estimates of each predictor

Article Snippet: In our previous report (Nagai et al. ), univariate analyses revealed that age, serum LDH levels, MRI findings, and endometrial cytology findings were significant factors in the preoperative prediction of uterine sarcoma.

Techniques:

Journal: SpringerPlus

Article Title: Highly improved accuracy of the revised PREoperative sarcoma score (rPRESS) in the decision of performing surgery for patients presenting with a uterine mass

doi: 10.1186/s40064-015-1318-7

Figure Lengend Snippet: The revised PREoperative sarcoma scoring system (rPRESS)

Article Snippet: In our previous report (Nagai et al. ), univariate analyses revealed that age, serum LDH levels, MRI findings, and endometrial cytology findings were significant factors in the preoperative prediction of uterine sarcoma.

Techniques: